The Environmental Monitoring Program (EMP) began in 1975 to conduct baseline and compliance monitoring of water quality, phytoplankton, zooplankton, and benthic invertebrates in the San Francisco Bay-Delta estuary. This monitoring program was designed to track the impact of water diversions to the State Water Project (SWP) and Central Valley Project (CVP) on the Bay-Delta. In the decades since, EMP scientists have monitored these constituents at fixed and floating stations throughout the estuary and ensured compliance with state and federal mandates such as Water Right Decision 1641 (D-1641). In the years and decades since its inception, EMP has become one of the cornerstones for scientists' and managers' understanding of the pace and pattern of change in this critical ecosystem. By sampling water quality and biological communities concurrently, EMP has created a dataset that is uniquely useful in better understanding causal connections between physical, biological, and biogeochemical processes.
The Sacramento-San Joaquin Delta (Delta) is experiencing an increase in the frequency and severity of Cyanobacterial Harmful Algal Blooms (CHABs), which can produce harmful cyanotoxins. This issue is likely to intensify due to climate changes and rising temperatures. The most common CHAB genus in the Delta is Microcystis. Currently, the most extensive dataset for tracking Delta CHABs is the Microcystis Visual Index (MVI), a qualitative assessment of Microcystis colony densities observed in surface water. This index, recorded by natural agency staff across numerous monitoring stations, provides broad spatial coverage but is inherently subjective and not quantitative, thereby limiting its utility.
This project has the following objectives: 1. Develop an MVI image classification model and model algorithm that can identify and quantify Microcystis aggregate presence and coverage level in digital photos. 2. Translate MVI rankings to Microcystis biomass ranges by obtaining data to ground-truth a range of Microcystis biomass that corresponds with MVI rankings 2 through 5. 3. Explore relationship between proportion of toxic Microcystis cells and Microcystis biomass levels by relating each MVI scale (for ranks 2 through 5) and Microcystis biomass range to a) proportion of toxic Microcystis cells (i.e. ratio of mcyE and 16S rDNA genes) and b) microcystin concentration, in surface grab samples.
